Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HEK-293 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33216-1-AP targets ZNF551 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35151 Product name: Recombinant human ZNF551 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 72-293 aa of BC005868 Sequence: QVRTSKGNTPTQKTHLSEIKMCVPVLKDILPAAEHQTTSPVQKSYLGSTSMRGFCFSADLHQHQKHYNEEEPWKRKVDEATFVTGCRFHVLNYFTCGEAFPAPTDLLQHEATPSGEEPHSSSSKHIQAFFNAKSYYKWGEYRKASSHKHTLVQHQSVCSEGGLYECSKCEKAFTCKNTLVQHQQIHTGQKMFECSECEESFSKKCHLILHKIIHTGERPYEC 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 551 |
| Calculated molecular weight | 670 aa, 78 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC005868 |
| Gene Symbol | ZNF551 |
| Gene ID (NCBI) | 90233 |
| RRID | AB_3742900 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q7Z340 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF551 encodes zinc finger protein 551, a KRAB domain-containing transcription factor with C2H2 zinc-finger domains that binds DNA and regulates RNA polymerase II-dependent transcription. It is predicted to act in the nucleus and to participate in regulation of gene expression, with activity tied to negative regulation of DNA-templated transcription and broader transcriptional control.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF551 antibody 33216-1-AP | Download protocol |
| WB protocol for ZNF551 antibody 33216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |