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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF543 Polyclonal antibody (21365-1-AP)

    WB analysis using 21365-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21365-1-AP (ZNF543 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZNF543 Polyclonal antibody

ZNF543 Polyclonal Antibody for WB, ELISA
Cat No. 21365-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21365-1-AP targets ZNF543 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF543 fusion protein Ag15830 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 543
Calculated molecular weight 600 aa, 68 kDa
Observed molecular weight68 kDa
GenBank accession numberBC101015
Gene Symbol ZNF543
Gene ID (NCBI) 125919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08ER8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF543 is a member of the Kruppel-associated box (KRAB) containing zinc finger proteins family, which function as transcription factors. ZNF543 has a wide range of physiological functions in a variety of cellular processes, which including apoptosis, cell proliferation and differentiation (PMID: 35467255, PMID: 34975305).

Protocols

Product Specific Protocols
WB protocol for ZNF543 antibody 21365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols