Validation Data Gallery
Tested Applications
| Positive WB detected in | A172 cells, U-118 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21317-1-AP targets ZNF536 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15971 Product name: Recombinant human ZNF536 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 949-1298 aa of BC150171 Sequence: VHGVDGGEEKPSGKSSQRKSEKSQYEPLDLSVRPDAASLPGSSVTVQDSIAWHGCLFCAFTTSSMELMALHLQANHLGKAKRKDNTIGVTVNCKDQAREASKMALLPSLQSNKDLGLSNMISSLDSASEKMAQGQLKETLGEQKSGAWTGHVDPAFCNFPSDFYKQFGVYPGMVGSGASSSCPNKEPDGKAHSEEDVPILIPETTSKNTTDDLSDIASSEDMDSSKGENNDEEDVETEPEMMTKPLSALSKDSSSDGGDSLQPTGTSQPVQGLVSPLSQAPEKQWHSQGLLQAQDPLAGLPKPERGPQSLDKPMNMLSVLRAYSSDGLAAFNGLASSTANSGCIKRPDLW 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 536 |
| Calculated molecular weight | 1300 aa, 141 kDa |
| Observed molecular weight | 130~150 kDa |
| GenBank accession number | BC150171 |
| Gene Symbol | ZNF536 |
| Gene ID (NCBI) | 9745 |
| RRID | AB_3742027 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15090 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF536 (Zinc finger protein 536) is a highly conserved zinc finger protein, acting as DNA-binding transcription suppressor, and negatively regulating neuron differentiation. ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal root ganglia and localized in the cerebral cortex, hippocampus, and hypothalamic area (PMID: 19398580).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF536 antibody 21317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |