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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZNF451 Polyclonal antibody

ZNF451 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25228-1-AP

Synonyms

COASTER, KIAA0576, KIAA1702, zinc finger protein 451, ZNF451

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 25228-1-AP

    WB analysis of HeLa using 25228-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25228-1-AP

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • IP experiment of HeLa using 25228-1-AP

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • IP experiment of HeLa using 25228-1-AP

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 4000ug.

  • IHC staining of human placenta using 25228-1-AP

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 25228-1-AP

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25228-1-AP

    IF Staining of HeLa using 25228-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25228-1-AP is ZNF451 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF451 fusion protein Ag18346 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 451
Calculated molecular weight 1061 aa, 121 kDa
Observed molecular weight 121 kDa
GenBank accession numberBC021712
Gene symbol ZNF451
Gene ID (NCBI) 26036
RRIDAB_2879975
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF451 antibody 25228-1-APDownload protocol
IHC protocol for ZNF451 antibody 25228-1-APDownload protocol
IF protocol for ZNF451 antibody 25228-1-APDownload protocol
IP protocol for ZNF451 antibody 25228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
  • KD Validated
View Article