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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    WB analysis of SH-SY5Y using 16218-1-AP (same clone as 16218-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    WB analysis of SH-SY5Y using 16218-1-AP (same clone as 16218-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    IF Staining of SH-SY5Y using 16218-1-AP (same clone as 16218-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 16218-1-AP (ZNF446 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16218-1-PBS in a different storage buffer formulation.

ZNF446 Polyclonal antibody, PBS Only

ZNF446 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16218-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16218-1-PBS targets ZNF446 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9266

Product name: Recombinant human ZNF446 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-422 aa of BC017206

Sequence: EQFLGTLPPEIQAWVRGQRPGSPEEAAALVEGLQHDPGQLLGWITAHVLKQEVLPAAQKTEEPLGSPHPSGTVESPGEGPQDTRIEGSVQLSCSVKEEPNVDGQEVAPSSPPLAAQSPEGHHGHQEPASTSFHPPRIQEEWGLLDRSQKELYWDAMLEKYGTVVSLGLPPHQPEAQAQSELGMLLTGTGVCRSLRSGNESEGPPGCPEAQPPQGPGPAAWEGLSGAATPAPTVRPGTPPVPTQPTPAETRLEPAATPRKPYTCEQCGRGFDWKSVFVIHHRTHTSGPGVQSPGLATGESTEKPPQGEVAFPHHPRHSLTGPRSYPCEECGCSFSWKSQLVIHRKSHRPEVP

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 446
Calculated molecular weight 422 aa, 46 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC017206
Gene Symbol ZNF446
Gene ID (NCBI) 55663
RRIDAB_2878231
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWS9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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