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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF410 Polyclonal antibody (14529-1-AP)

    WB analysis using 14529-1-AP (same clone as 14529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZNF410 Polyclonal antibody (14529-1-AP)

    WB analysis using 14529-1-AP (same clone as 14529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14529-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ZNF410 Polyclonal antibody, PBS Only

ZNF410 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14529-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Zinc finger protein APA 1, Zinc finger protein 410, APA1, APA 1, Another partner for ARF 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14529-1-PBS targets ZNF410 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF410 fusion protein Ag6028 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 410
Calculated molecular weight 52 kDa
Observed molecular weight52-67 kDa
GenBank accession numberBC050683
Gene Symbol ZNF410
Gene ID (NCBI) 57862
RRIDAB_2257520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VK4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)