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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of human brain using 11759-1-AP (same clone as 11759-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZNF395 Polyclonal antibody (11759-1-AP)

    WB analysis of HeLa using 11759-1-AP (same clone as 11759-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11759-1-AP (ZNF395 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ZNF395 Polyclonal antibody (11759-1-AP)

    IHC staining of human pancreas cancer using 11759-1-AP (same clone as 11759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11759-1-AP (ZNF395 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF395 Polyclonal antibody (11759-1-AP)

    IF Staining of HepG2 using 11759-1-AP (same clone as 11759-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF395 antibody (11759-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11759-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZNF395 Polyclonal antibody, PBS Only

ZNF395 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11759-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HD-regulating factor 2, HDBP-2, HDBP2, HDBP 2, HD regulating factor 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11759-1-PBS targets ZNF395 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2355

Product name: Recombinant human ZNF395 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 161-513 aa of BC001237

Sequence: DAVEMDEMMAAMVLTSLSCSPVVQSPPGTEANFSASRAACDPWKESGDISDSGSSTTSGHWSGSSGVSTPSPPHPQASPKYLGDAFGSPQTDHGFETDPDPFLLDEPAPRKRKNSVKVMYKCLWPNCGKVLRSIVGIKRHVKALHLGDTVDSDQFKREEDFYYTEVQLKEESAAAAAAAAAGTPVPGTPTSEPAPTPSMTGLPLSALPPPLHKAQSSGPEHPGPESSLPSGALSKSAPGSFWHIQADHAYQALPSFQIPVSPHIYTSVSWAAAPSAACSLSPVRSRSLSFSEPQQPAPAMKSHLIVTSPPRAQSGARKARGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLD

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 395
Calculated molecular weight 513 aa, 55 kDa
Observed molecular weight 62 kDa, 72 kDa
GenBank accession numberBC001237
Gene Symbol ZNF395
Gene ID (NCBI) 55893
RRIDAB_2218065
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8N7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].

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