Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, HepG2 cells, mouse spleen tissue |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25785-1-AP targets ZNF394 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22655 Product name: Recombinant human ZNF394 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-375 aa of BC051847 Sequence: RALQRALDGTSSQGMVTFEDTAVSLTWEEWERLDPARRDFCRESAQKDSGSTVPPSLESRVENKELIPMQQILEEAEPQGQLQEAFQGKRPLFSKCGSTHEDRVEKQSGDPLPLKLENSPEAEGLNSISDVNKNGSIEGEDSKNNELQNSARCSNLVLCQHIPKAERPTDSEEHGNKCKQSFHMVTWHVLKPHKSDSGDSFHHSSLFETQRQLHEERPYKCGNCGKSFKQRSDLFR 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 394 |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | BC051847 |
| Gene Symbol | ZNF394 |
| Gene ID (NCBI) | 84124 |
| RRID | AB_2880239 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53GI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF394 (zinc finger protein 394) is a C2H2-type zinc finger protein belonging to the Krüppel C2H2-type zinc finger protein family. The protein contains three distinct domains: the N-terminus has two domains, LER and KRAB, and the C-terminus has seven C2H2 zinc finger domains. ZNF394 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZNF394, together with ZIM3, plays a key role in human embryonic development at the 8-cell stage by inhibiting the expression of genes specific to the 4-cell stage, thereby promoting embryonic development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF394 antibody 25785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
