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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF281 Polyclonal antibody (24088-1-AP)

    WB analysis using 24088-1-AP (same clone as 24088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24088-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZNF281 Polyclonal antibody (24088-1-AP)

    IF Staining of U2OS using 24088-1-AP (same clone as 24088-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24088-1-PBS in a different storage buffer formulation.

ZNF281 Polyclonal antibody, PBS Only

ZNF281 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24088-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GC-box-binding zinc finger protein 1, GZP1, Transcription factor ZBP-99, ZBP 99, ZBP99

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24088-1-PBS targets ZNF281 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21355

Product name: Recombinant human ZNF281 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 695-895 aa of BC060820

Sequence: LSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 281
Calculated molecular weight 895 aa, 97 kDa
Observed molecular weight110 kDa
GenBank accession numberBC060820
Gene Symbol ZNF281
Gene ID (NCBI) 23528
RRIDAB_3085722
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y2X9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF281 (zinc finger protein 281) is a Krüppel family transcription factor containing four C2H2-type zinc finger domains, located in the cell nucleus. It promotes epithelial-mesenchymal transition (EMT) and tumor metastasis by recruiting co-repressors such as CTBP or synergistically with ZEB1 to inhibit the expression of epithelial markers such as E-cadherin. Furthermore, this protein is also involved in maintaining embryonic stem cell pluripotency and regulating neural differentiation.

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