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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF281 Polyclonal antibody (24088-1-AP)

    WB analysis using 24088-1-AP (same clone as 24088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24088-1-AP (ZNF281 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24088-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ZNF281 Polyclonal antibody (24088-1-AP)

    IF Staining of U2OS using 24088-1-AP (same clone as 24088-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF281 antibody (24088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24088-1-PBS in a different storage buffer formulation.

ZNF281 Polyclonal antibody, PBS Only

ZNF281 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24088-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GC-box-binding zinc finger protein 1, GZP1, Transcription factor ZBP-99, ZBP 99, ZBP99

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24088-1-PBS targets ZNF281 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21355

Product name: Recombinant human ZNF281 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 695-895 aa of BC060820

Sequence: LSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 281
Calculated molecular weight 895 aa, 97 kDa
Observed molecular weight110 kDa
GenBank accession numberBC060820
Gene Symbol ZNF281
Gene ID (NCBI) 23528
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y2X9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF281 (zinc finger protein 281) is a Krüppel family transcription factor containing four C2H2-type zinc finger domains, located in the cell nucleus. It promotes epithelial-mesenchymal transition (EMT) and tumor metastasis by recruiting co-repressors such as CTBP or synergistically with ZEB1 to inhibit the expression of epithelial markers such as E-cadherin. Furthermore, this protein is also involved in maintaining embryonic stem cell pluripotency and regulating neural differentiation.

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