Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, U2OS cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32519-1-AP targets ZNF280B in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38544 Product name: Recombinant human ZNF280B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 75-268 aa of NM_080764.3 Sequence: YDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRANELAKTDILSLT 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 280B |
| Calculated molecular weight | 62kDa，543aa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | NM_080764.3 |
| Gene Symbol | ZNF280B |
| Gene ID (NCBI) | 140883 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86YH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF280B belongs to the family of zinc finger proteins, a transcription factor that is mainly involved in the regulation of gene expression. In prostate cancer cells, ZNF280B induces p53 nuclear export, leading to subsequent proteasomal degradation. Therefore, ZNF280B has pro-growth and pro-survival functions in prostate cancer cells. In addition to this, ZNF280B has a promotional role in gastric and lung cancer development, and its potential as a tumor therapeutic target can be explored in the future.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ZNF280B antibody 32519-1-AP | Download protocol |
| WB protocol for ZNF280B antibody 32519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |