Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse adrenal gland tissue, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31903-1-AP targets ZNF280A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35942 Product name: Recombinant human ZNF280A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 461-542 aa of BC053901 Sequence: LTLKEEIEHKTKDHQTFKKPEQLQGLPSETKVIIQTSVQPGSSGMASVIVSNTDPQSSPVKTKKKTAMNTRDSRLPCSKDSS 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 280A |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC053901 |
| Gene Symbol | ZNF280A |
| Gene ID (NCBI) | 129025 |
| RRID | AB_3670138 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P59817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc finger proteins are the largest transcription factor family in the human genome and play a significant role in regulating and managing gene expression, including development, differentiation, metabolism, and autophagy (PMID: 18253864; 27411336). Zinc finger protein 280A (ZNF280A, also known as SUHW1, ZNF280, and ZNF636) is a member of the zinc-finger protein family, carrying two consecutive Cys2His2 zinc finger domains (PMID: 33414445). Cys2-His2 (C2H2) zinc finger domains (ZFs) were originally identified as DNA-binding domains, and uncharacterized domains are typically assumed to function in DNA binding (PMID: 18253864).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF280A antibody 31903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |