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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using ZNF271 Polyclonal antibody (23799-1-AP)

    WB analysis of mouse pancreas using 23799-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 23799-1-AP (ZNF271 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF271 Polyclonal antibody

ZNF271 Polyclonal Antibody for WB, ELISA
Cat No. 23799-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HZF7, zinc finger protein 271, ZNF271, ZNFEB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23799-1-AP targets ZNF271 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20727

Product name: Recombinant human ZNF271 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 72-423 aa of BC017710

Sequence: IRGFILERNPIPVINVAKPSVGFQILLIINEFTLERSLTHVISAIKCLVEDEILLNITEFIQVRNPMNVMNVGKPLVRAPTLFFIRESTLERNLMHVVIVLKALVAVQILLSIKEYTLERNHMHVISVIKVLVKAQTSLNIREYTLVKSLIIAIVVRKPSVRVLTLFFIREFTLEKNYYLCTQCSKSFSQISDLIKHQRIHTGEKPYKCSECRKAFSQCSALTLHQRIHTGKKPNPCDECGKSFSRRSDLINHQKIHTGEKPYKCDACGKAFSTCTDLIEHQKTHAEEKPYQCVQCSRSCSQLSELTIHEEVHCGEDSQNVMNVRKPLVCTPTLFSTRDTVPEKNLMNAVDY

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 271
Calculated molecular weight 76 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC017710
Gene Symbol ZNF271
Gene ID (NCBI) 10778
RRIDAB_2879327
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF271, also named as ZNF-EB, HZF7, ZNF271-dp, may be involved in transcriptional regulation. It plays a role in the EBV-mediated transformation process. ZNF271 has two isoforms with MW 76 kDa and 48 kDa. This antibody recognize both isoforms.

Protocols

Product Specific Protocols
WB protocol for ZNF271 antibody 23799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols