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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis of HEK-293 using 67730-1-Ig

    WB result of ZNF217 antibody (67730-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis of HEK-293 using 67730-1-Ig

    WB result of ZNF217 antibody (67730-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

  • Western Blot (WB) analysis of 4T1 cells using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis of 4T1 using 67730-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZNF217 Monoclonal antibody (67730-1-Ig)

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZNF217 Monoclonal antibody (67730-1-Ig)

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

ZNF217 Monoclonal antibody

ZNF217 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67730-1-Ig
Clone No.1F11B6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ZFP217, 1F11B6, ZABC1, Zinc finger protein 217

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, 4T1 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, PC-3 cells, K-562 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67730-1-Ig targets ZNF217 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19468

Product name: Recombinant human ZNF217 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 707-1043 aa of BC113427

Sequence: IPSITCPFCTFKTFYPEVLMMHQRLEHKYNPDVHKNCRNKSLLRSRRTGCPPALLGKDVPPLSSFCKPKPKSAFPAQSKSLPSAKGKQSPPGPGKAPLTSGIDSSTLAPSNLKSHRPQQNVGVQGAATRQQQSEMFPKTSVSPAPDKTKRPETKLKPLPVAPSQPTLGSSNINGSIDYPAKNDSPWAPPGRDYFCNRSASNTAAEFGEPLPKRLKSSVVALDVDQPGANYRRGYDLPKYHMVRGITSLLPQDCVYPSQALPPKPRFLSSSEVDSPNVLTVQKPYGGSGPLYTCVPAGSPASSSTLEGKRPVSYQHLSNSMAQKRNYENFIGNAHYRP

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 217
Calculated molecular weight 1048 aa, 115 kDa
Observed molecular weight110-130 kDa
GenBank accession numberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_2882916
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO75362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF217 antibody 67730-1-IgDownload protocol
IHC protocol for ZNF217 antibody 67730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols