Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue | 
| Positive IF/ICC detected in | HepG2 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25502-1-AP targets ZNF215 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag22026 Product name: Recombinant human ZNF215 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 110-304 aa of BC014538 Sequence: LQHPNNSKDMVTLIEDVIEMLEDEDMPCKDSALQMGSIKEKMKAGSRTGKPQEPVTFKDVVVEFSKEEWGQLDSAVKNLYRNVMLENFRNLNSLRKAHLLSKPFESLKLESKKKRWIMEKEIPRKTIFDMKSISGEESSHGVIMTRLTESGHPSSDAWKDGVSLLLPRLEYNDAVSAHCNLHLWVQVILLPQPPE相同性解析による交差性が予測される生物種 | 
| Full Name | zinc finger protein 215 | 
| Calculated molecular weight | 517 aa, 60 kDa | 
| Observed molecular weight | 55-60 kDa | 
| GenBank accession number | BC014538 | 
| Gene Symbol | ZNF215 | 
| Gene ID (NCBI) | 7762 | 
| RRID | AB_2880105 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q9UL58 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNF215 antibody 25502-1-AP | Download protocol | 
| WB protocol for ZNF215 antibody 25502-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 


