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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF189 Polyclonal antibody (26169-1-AP)

    WB analysis using 26169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26169-1-AP (ZNF189 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF189 Polyclonal antibody (26169-1-AP)

    IF Staining of HeLa using 26169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF189 antibody (26169-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ZNF189 Polyclonal antibody

ZNF189 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26169-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger protein 189

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, THP-1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26169-1-AP targets ZNF189 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF189 fusion protein Ag23264 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 189
Observed molecular weight68 kDa
GenBank accession numberBC126120
Gene Symbol ZNF189
Gene ID (NCBI) 7743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75820
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF189 antibody 26169-1-APDownload protocol
IF protocol for ZNF189 antibody 26169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FEBS J

Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

Authors - Xiao Li
View Article