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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using ZNF140 Polyclonal antibody (24531-1-AP)

    WB analysis of SKOV-3 using 24531-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF140 Polyclonal antibody (24531-1-AP)

    WB analysis of Jurkat using 24531-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF140 Polyclonal antibody (24531-1-AP)

    IF Staining of HepG2 using 24531-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF140 antibody (24531-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ZNF140 Polyclonal antibody

ZNF140 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24531-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

pHZ 39, Zinc finger protein 140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24531-1-AP targets ZNF140 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21379

Product name: Recombinant human ZNF140 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 79-162 aa of BC040561

Sequence: SESSGEIKDFSPKNVIYDDSSQYLIMERILSQGPVYSSFKGGWKCKDHTEMLQENQGCIRKVTVSHQEALAQHMNISTVERPYG

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 140
Calculated molecular weight 457 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC040561
Gene Symbol ZNF140
Gene ID (NCBI) 7699
RRIDAB_2879592
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52738
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZNF140 antibody 24531-1-APDownload protocol
IF protocol for ZNF140 antibody 24531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols