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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ZNF132 Polyclonal antibody (21681-1-AP)

    WB analysis of mouse heart using 21681-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21681-1-AP (ZNF132 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ZNF132 Polyclonal antibody (21681-1-AP)

    WB analysis of human testis using 21681-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 21681-1-AP (ZNF132 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ZNF132 Polyclonal antibody

ZNF132 Polyclonal Antibody for WB, ELISA
Cat No. 21681-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

pHZ 12, zinc finger protein 132, ZNF132

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse heart tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21681-1-AP targets ZNF132 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16142

Product name: Recombinant human ZNF132 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 299-602 aa of BC109107

Sequence: AFSHSSKLRKHQKFHTEVKYYECIACGKTFNHKLTFVHHQRIHSGERPYECDECGKAFSNRSHLIRHEKVHTGERPFECLKCGRAFSQSSNFLRHQKVHTQVRPYECSQCGKSFSRSSALIQHWRVHTGERPYECSECGRAFNNNSNLAQHQKVHTGERPFECSECGRDFSQSSHLLRHQKVHTGERPFECCDCGKAFSNSSTLIQHQKVHTGQRPYECSECRKSFSRSSSLIQHWRIHTGEKPYECSECGKAFAHSSTLIEHWRVHTKERPYECNECGKFFSQNSILIKHQKVHTGEKPYKCS

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 132
Calculated molecular weight 706 aa, 81 kDa
Observed molecular weight 81 and 68 kDa
GenBank accession numberBC109107
Gene Symbol ZNF132
Gene ID (NCBI) 7691
RRIDAB_2878903
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52740
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZNF132, also named as Zinc finger protein 132, is a 706 amino-acid protein, which contains 18 C2H2-type zinc fingers and 1 KRAB domain. ZNF132 belongs to the krueppel C2H2-type zinc-finger protein family and localizes in the nucleus. ZNF132 may be involved in transcriptional regulation. ZNF132 exists as two alternatively spliced isoforms, and is encoded by a gene that maps to human chromosome 19.

Protocols

Product Specific Protocols
WB protocol for ZNF132 antibody 21681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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