Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of MCF-7 using 11633-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 11633-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11633-1-AP
IP result of anti-ZMYND8 (IP:11633-1-AP, 3ug; Detection:11633-1-AP 1:300) with HeLa cells lysate 920ug.
IHC staining of human lung cancer using 11633-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11633-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11633-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11633-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ZMYND8 Polyclonal antibody

ZMYND8 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11633-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

CTCL associated antigen se14 3, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, KIAA1125, MYND-type zinc finger-containing chromatin reader ZMYND8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 1 publications below
ChIP	See 1 publications below

Product Information

11633-1-AP targets ZMYND8 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2207 Product name: Recombinant human ZMYND8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 745-1094 aa of BC030721 Sequence: WNSSTVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD 相同性解析による交差性が予測される生物種
Full Name	zinc finger, MYND-type containing 8
Calculated molecular weight	1186 aa, 132 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC030721
Gene Symbol	ZMYND8
Gene ID (NCBI)	23613
RRID	AB_2241834
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9ULU4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins, including REST corepressors (RCOR). ZMYND8 binds to ROCR2 and can function as a transcriptional repressor. It is co-expressed with RCOR2 in the nervous system, and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

Protocols

Product Specific Protocols
IHC protocol for ZMYND8 antibody 11633-1-AP	Download protocol
IP protocol for ZMYND8 antibody 11633-1-AP	Download protocol
WB protocol for ZMYND8 antibody 11633-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer Authors - Rashmi Bharti View Article
mouse,human		Mol Cell The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability. Authors - Qiang Pan KO Validated View Article
human	WB	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin KD Validated View Article
human	WB	Cancer Res KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity Authors - Yulong Qiang View Article
human	WB	Mol Cell Basis of gene-specific transcription regulation by the Integrator complex Authors - Kevin Sabath View Article
human	WB	Nat Cell Biol Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection Authors - Yixin Yin KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 11633-1-AP

WB analysis of HeLa using 11633-1-AP

IP experiment of HeLa using 11633-1-AP

IHC staining of human lung cancer using 11633-1-AP

IHC staining of human lung cancer using 11633-1-AP

IHC staining of human lung cancer using 11633-1-AP

IHC staining of human lung cancer using 11633-1-AP

ZMYND8 Polyclonal antibody

ZMYND8 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity

Basis of gene-specific transcription regulation by the Integrator complex

Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection