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Tested Applications
| Positive WB detected in | SK-BR-3 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16159-1-AP targets ZMIZ1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, MIZ-type containing 1 |
| Calculated molecular weight | 115 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | NM_020338 |
| Gene Symbol | ZMIZ1 |
| Gene ID (NCBI) | 57178 |
| RRID | AB_3669232 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZMIZ2 and ZMIZ1 are members of a PIAS-like family of proteins that interact with nuclear hormone receptors. It can act as a transcriptional coactivator, increasing ligand-dependent transcriptional activity of AR and promoting AR sumoylation. The stimulation of AR activity is dependent upon sumolylation. This antibody is specific for ZMIZ1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZMIZ1-Specific antibody 16159-1-AP | Download protocol |
| WB protocol for ZMIZ1-Specific antibody 16159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |