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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZMIZ1-Specific Polyclonal antibody (16159-1-AP)

    WB analysis using 16159-1-AP

    SK-BR-3 cell lysates were subjected to SDS PAGE followed by western blot with 16159-1-AP (ZMIZ1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ZMIZ1-Specific Polyclonal antibody (16159-1-AP)

    IF Staining of A549 using 16159-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZMIZ1-Specific antibody (16159-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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ZMIZ1-Specific Polyclonal antibody

ZMIZ1-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16159-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZMIZ1, RAI17, PIAS-like protein Zimp10, PIAS like protein Zimp10, MIZ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSK-BR-3 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16159-1-AP targets ZMIZ1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name zinc finger, MIZ-type containing 1
Calculated molecular weight 115 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_020338
Gene Symbol ZMIZ1
Gene ID (NCBI) 57178
RRIDAB_3669232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZMIZ2 and ZMIZ1 are members of a PIAS-like family of proteins that interact with nuclear hormone receptors. It can act as a transcriptional coactivator, increasing ligand-dependent transcriptional activity of AR and promoting AR sumoylation. The stimulation of AR activity is dependent upon sumolylation. This antibody is specific for ZMIZ1.

Protocols

Product Specific Protocols
WB protocol for ZMIZ1-Specific antibody 16159-1-APDownload protocol
IF protocol for ZMIZ1-Specific antibody 16159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols