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Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using ZIM3 Polyclonal antibody (25200-1-AP)

    WB analysis of COLO 320 using 25200-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25200-1-AP (ZIM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ZIM3 Polyclonal antibody (25200-1-AP)

    WB analysis of A549 using 25200-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25200-1-AP (ZIM3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZIM3 Polyclonal antibody

ZIM3 Polyclonal Antibody for WB, ELISA
Cat No. 25200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZIM 3, Zinc finger protein 657, ZNF657

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inCOLO 320 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25200-1-AP targets ZIM3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18411

Product name: Recombinant human ZIM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 56-327 aa of BC114503

Sequence: DVILRLEQGKEPWLEEEEVLGSGRAEKNGDIGGQIWKPKDVKESLAREVPSINKETLTTQKGVECDGSKKILPLGIDDVSSLQHYVQNNSHDDNGYRKLVGNNPSKFVGQQLKCNACRKLFSSKSRLQSHLRRHACQKPFECHSCGRAFGEKWKLDKHQKTHAEERPYKCENCGNAYKQKSNLFQHQKMHTKEKPYQCKTCGKAFSWKSSCINHEKIHNAKKSYQCNECEKSFRQNSTLIQHKKVHTGQKPFQCTDCGKAFIYKSDLVKHQR

相同性解析による交差性が予測される生物種
Full Name zinc finger, imprinted 3
Calculated molecular weight 472 aa, 57 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC114503
Gene Symbol ZIM3
Gene ID (NCBI) 114026
RRIDAB_2879955
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZIM3 (Zinc Finger Imprinted 3), also known as ZNF657 or ZNF264, is a protein containing zinc finger and KRAB domains. It belongs to the Krüppel C2H2-type zinc finger protein family and comprises 11 C2H2-type zinc fingers and 1 KRAB domain. ZIM3 influences chromatin organization by binding to the KAP1 protein, thereby regulating gene transcription. ZIM3 is predicted to have DNA-binding transcription factor activity and is involved in the transcriptional regulation of RNA polymerase II. Additionally, ZIM3 plays a core regulatory role in human major zygotic genome activation (ZGA).

Protocols

Product Specific Protocols
WB protocol for ZIM3 antibody 25200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols