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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZIC4-Specific Polyclonal antibody

ZIC4-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51003-1-AP

Synonyms

Zic family member 4, ZIC4, Zinc finger protein ZIC 4

Validation Data Gallery

Applications:
  • WB analysis of human brain using 51003-1-AP

    WB analysis of human brain using 51003-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 51003-1-AP

    IHC staining of mouse brain using 51003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 51003-1-AP

    IHC staining of mouse brain using 51003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51003-1-AP (ZIC4-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

51003-1-AP targets ZIC4-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Zic family member 4
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberNM_032153
Gene symbol ZIC4
Gene ID (NCBI) 84107
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The ZIC gene family includes 5 genes that are highly conserved across evolution. These genes encode zinc finger proteins that are expressed during development and maturation of the central nervous system and have critical roles in the development of the cerebellum. ZIC4, likes other member, may has roles in the nervous system development.

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Neural Tube Defects and ZIC4 Hypomethylation in Relation to Polycyclic Aromatic Hydrocarbon Exposure.

Authors - Yun Huang
View Article
humanWB,IHC

BMC Cancer

Expression of ZIC family genes in meningiomas and other brain tumors.

Authors - Aruga Jun J
View Article