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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of Neuro-2a using 21717-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using ZIC3 Polyclonal antibody (21717-1-AP)

    WB analysis of Y79 using 21717-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of rat brain using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of rat cerebellum using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of human urothelial carcinoma using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of mouse cerebellum using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of human thyroid cancer using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ZIC3 Polyclonal antibody (21717-1-AP)

    IHC staining of mouse brain using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZIC3 Polyclonal antibody

ZIC3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21717-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ZNF203, Zinc finger protein ZIC 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNeuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21717-1-AP targets ZIC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16354

Product name: Recombinant human ZIC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC113395

Sequence: MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYR

相同性解析による交差性が予測される生物種
Full Name Zic family member 3 (odd-paired homolog, Drosophila)
Calculated molecular weight 467 aa, 51 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC113395
Gene Symbol ZIC3
Gene ID (NCBI) 7547
RRIDAB_2878910
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60481
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ZIC3 antibody 21717-1-APDownload protocol
WB protocol for ZIC3 antibody 21717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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