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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZIC3 Polyclonal antibody

ZIC3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21717-1-AP

Synonyms

HTX, HTX1, ZIC3, Zinc finger protein ZIC 3, ZNF203

Validation Data Gallery

Applications:
  • WB analysis of Neuro-2a using 21717-1-AP

    WB analysis of Neuro-2a using 21717-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 21717-1-AP

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 21717-1-AP

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 21717-1-AP

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse cerebellum using 21717-1-AP

    WB analysis of mouse cerebellum using 21717-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 21717-1-AP

    WB analysis of SH-SY5Y using 21717-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 21717-1-AP

    WB analysis of Y79 using 21717-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNeuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy ZIC3 Ready-To-Use IHC Kit. ZIC3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21717-1-AP is ZIC3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZIC3 fusion protein Ag16354 相同性解析による交差性が予測される生物種
Full Name Zic family member 3 (odd-paired homolog, Drosophila)
Calculated molecular weight 467 aa, 51 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC113395
Gene symbol ZIC3
Gene ID (NCBI) 7547
RRIDAB_2878910
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZIC3 antibody 21717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other