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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis using 13903-1-AP (same clone as 13903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of mouse kidney using 13903-1-AP (same clone as 13903-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of mouse heart using 13903-1-AP (same clone as 13903-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZHX1 Polyclonal antibody (13903-1-AP)

    WB analysis of HeLa using 13903-1-AP (same clone as 13903-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13903-1-AP (ZHX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZHX1 Polyclonal antibody (13903-1-AP)

    IP experiment of HEK-293 using 13903-1-AP (same clone as 13903-1-PBS)

    IP result of anti-ZHX1 (IP:13903-1-AP, 4ug; Detection:13903-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 13903-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZHX1 Polyclonal antibody, PBS Only

ZHX1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13903-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

zinc fingers and homeoboxes 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13903-1-PBS targets ZHX1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4874

Product name: Recombinant human ZHX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 524-873 aa of BC040481

Sequence: SNQCLHLNNDSSTTIIIDSSDETTESPTVGTAQPKQSWNPFPDFTPQKFKEKTAEQLRVLQASFLNSSVLTDEELNRLRAQTKLTRREIDAWFTEKKKSKALKEEKMEIDESNAGSSKEEAGETSPADESGAPKSGSTGKICKKTPEQLHMLKSAFVRTQWPSPEEYDKLAKESGLARTDIVSWFGDTRYAWKNGNLKWYYYYQSANSSSMNGLSSLRKRGRGRPKGRGRGRPRGRPRGSKRINNWDRGPSLIKFKTGTAILKDYYLKRKFLNEQDLDELVNKSHMGYEQVREWFAERQRRSELGIELFEENEEEDEVIDDQEEDEEETDDSDTWEPPRHVKRKLSKSDD

相同性解析による交差性が予測される生物種
Full Name zinc fingers and homeoboxes 1
Calculated molecular weight 873 aa, 98 kDa
Observed molecular weight 100-120 kDa
GenBank accession numberBC040481
Gene Symbol ZHX1
Gene ID (NCBI) 11244
RRIDAB_2219214
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKY1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Binding of the nuclear factor-Y complex (NF-Y) to the inverted CCAAT-box interferes with transcription activation through nucleosome reorganization. The three homologous proteins forming the zinc-fingers and homeoboxes (ZHX) family interact with the activation domain of NF-Ya to repress transcription [PMID:19348505,20509910]. Human zinc-fingers and homeoboxes 1 (ZHX1) was cloned as a protein that interacts with the activation domain (AD) of the A subunit of nuclear factor-Y (NF-YA) [PMID:12237128].

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