Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, HepG2 cells, |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
23203-1-AP targets ZGPAT in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19620 Product name: Recombinant human ZGPAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 173-511 aa of BC032612 Sequence: THKSLKPCPFFLEGKCRFKENCRFSHGQVVSLDELRPFQDPDLSSLQAGSACLAKHQDGLWHAARITDVDNGYYTVKFDSLLLREAVVEGDGILPPLRTEATESDSDSDGTGDSSYARVVGSDAVDSGTCSSAFAGWEVHTRGIGSRLLTKMGYEFGKGLGRHAEGRVEPIHAVVLPRGKSLDQCVETLQKQTRVGKAGTNKPPRCRGRGARPGGRPAPRNVFDFLNEKLQGQAPGALEAGAAPAGRRSKDMYHASKSAKRALSLRLFQTEEKIERTQRDIRSIQEALARNAGRHSVASAQLQEKLAGAQRQLGQLRAQEAGLQQEQRKADTHKKMTEF 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, CCCH-type with G patch domain |
| Calculated molecular weight | 531 aa, 57 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC032612 |
| Gene Symbol | ZGPAT |
| Gene ID (NCBI) | 84619 |
| RRID | AB_2879231 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8N5A5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZGPAT (Zinc finger CCCH-type with G patch domain-containing protein), also known as zinc finger CCCH domain-containing protein 9 (ZC3HDC9) and G patch domain-containing protein 6 (GPATC6), is a 531 amino acid protein that contains a G-patch domain, which is typically found within RNA-binding proteins. Proteins that contain the G-patch domain include some tumor suppressor and DNA-damage repair proteins. ZGPAT also contains one C3H1-type zinc finger, which further supports its probable role as an RNA-binding protein. The gene encoding ZGPAT is inactivated via differential methylation in a oligodendroglioma cell line, suggesting that ZGPAT may have utility as a biomarker. There are several isoforms of ZGPAT that are produced as a result of alternative splicing events.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZGPAT antibody 23203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
