Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19796 Product name: Recombinant human ZFYVE28 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 575-887 aa of BC137309 Sequence: EDVVERLREKCSPGGVIGASYAAGLAKASDRAPERQEEAPPPSEDASNGREPKAPTSDKCLPHTSGSQVDTASGLQGEAGVAGQQEPEARELHAGSPPAHEAPQALSGSSSSTAGSCSSDKMGPEAAPAATHAAPQATREKIRSRFHGSHDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPETDDKEKLRKVTQTLRSAALEDCALCQETLSSSELAAKTRDGDFEDPPEWVPDEACGFCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKAGL 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, FYVE domain containing 28 |
| Calculated molecular weight | 887 aa, 96 kDa |
| Observed molecular weight | 96 kDa |
| GenBank accession number | BC137309 |
| Gene Symbol | ZFYVE28 |
| Gene ID (NCBI) | 57732 |
| RRID | AB_3085790 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZFYVE28 antibody 25360-1-AP | Download protocol |
| WB protocol for ZFYVE28 antibody 25360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
