Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 11 publications below |
| IF | See 4 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3369 Product name: Recombinant human ZFYVE27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-411 aa of BC030621 Sequence: VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, FYVE domain containing 27 |
| Calculated molecular weight | 411 aa, 46 kDa |
| Observed molecular weight | 46-50 kDa |
| GenBank accession number | BC030621 |
| Gene Symbol | ZFYVE27 |
| Gene ID (NCBI) | 118813 |
| RRID | AB_10640298 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T4F4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Protrudin antibody 12680-1-AP | Download protocol |
| WB protocol for Protrudin antibody 12680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS. | ||
Nat Commun PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. | ||
Nat Commun Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. | ||
Proc Natl Acad Sci U S A Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
| ||
Cell Mol Life Sci ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic. | ||
Cell Mol Life Sci Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
|