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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFYVE16 Polyclonal antibody (13118-2-AP)

    WB analysis using 13118-2-AP (same clone as 13118-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    WB analysis of mouse kidney using 13118-2-AP (same clone as 13118-2-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    WB analysis of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ZFYVE16 Polyclonal antibody (13118-2-AP)

    WB analysis of HEK-293 using 13118-2-AP (same clone as 13118-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ZFYVE16 Polyclonal antibody (13118-2-AP)

    WB analysis of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZFYVE16 Polyclonal antibody (13118-2-AP)

    IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZFYVE16 Polyclonal antibody, PBS Only

ZFYVE16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13118-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13118-2-PBS targets ZFYVE16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3811

Product name: Recombinant human ZFYVE16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC032227

Sequence: MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTSALTCSL

相同性解析による交差性が予測される生物種
Full Name zinc finger, FYVE domain containing 16
Calculated molecular weight 1539 aa, 169 kDa
Observed molecular weight230-250 kDa
GenBank accession numberBC032227
Gene Symbol ZFYVE16
Gene ID (NCBI) 9765
RRIDAB_2288795
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3T8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

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