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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    WB analysis of mouse liver using 28012-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZFPM1 Polyclonal antibody (28012-1-AP)

    WB analysis using 28012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    IHC staining of human prostate cancer using 28012-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFPM1 Polyclonal antibody (28012-1-AP)

    IHC staining of human prostate cancer using 28012-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZFPM1 Polyclonal antibody

ZFPM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28012-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FOG, FOG 1, FOG1, Friend of GATA 1, Friend of GATA protein 1, ZFPM1, Zinc finger protein ZFPM1, ZNF408, ZNF89A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28012-1-AP targets ZFPM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27829

Product name: Recombinant human ZFPM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-506 aa of NM_153813

Sequence: MTKANCERHLKVHTDTLSGVCHSCGFISTTRDILYSHLVTNHMVCQPGSKGEIYSPGAGHPATKLPPDSLGSFQQQHTALQGPLASADLGLAPTPSPGLDRKALAEATNGEARAEPLAQNGGSSEPPAAPRSIKVEAVEEPEAAPILGPGEPGPQAPSRTPSPRSPAPARVKAELSS

相同性解析による交差性が予測される生物種
Full Name zinc finger protein, multitype 1
Calculated molecular weight 105 aa
Observed molecular weight105 kDa
GenBank accession numberNM_153813
Gene Symbol ZFPM1
Gene ID (NCBI) 161882
RRIDAB_3086021
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IX07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).

Protocols

Product Specific Protocols
IHC protocol for ZFPM1 antibody 28012-1-APDownload protocol
WB protocol for ZFPM1 antibody 28012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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