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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZFPL1 Polyclonal antibody (27157-1-AP)

    WB analysis of HepG2 using 27157-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27157-1-AP (ZFPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFPL1 Polyclonal antibody (27157-1-AP)

    IF Staining of HepG2 using 27157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFPL1 antibody (27157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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ZFPL1 Polyclonal antibody

ZFPL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27157-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

D11S750, MCG4, ZFPL1, zinc finger protein like 1, Zinc finger protein MCG4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27157-1-AP targets ZFPL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFPL1 fusion protein Ag24097 相同性解析による交差性が予測される生物種
Full Name zinc finger protein-like 1
Observed molecular weight 35-40 kDa
GenBank accession numberBC012814
Gene Symbol ZFPL1
Gene ID (NCBI) 7542
RRIDAB_2880781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZFPL1 antibody 27157-1-APDownload protocol
IF protocol for ZFPL1 antibody 27157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols