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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFP64 Recombinant antibody (83825-3-RR)

    WB analysis using 83825-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83825-3-RR (ZFP64 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83825-3-RR

    Affinity and Kinetic Characterization of 83825-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83825-3-RR against Human ZFP64 were performed. The affinity constant is 1.53 nM.

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ZFP64 Recombinant antibody

ZFP64 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83825-3-RR
Clone No.240847F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ZNF338, Zinc finger protein 64, Zfp-64, Zfp 64, 240847F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, MKN-45 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83825-3-RR targets ZFP64 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZFP64 fusion protein Ag10971 相同性解析による交差性が予測される生物種
Full Name zinc finger protein 64 homolog (mouse)
Calculated molecular weight 681 aa, 75 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC041622
Gene Symbol ZFP64
Gene ID (NCBI) 55734
RRIDAB_3671410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NTW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.

Protocols

Product Specific Protocols
WB protocol for ZFP64 antibody 83825-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols