Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
17187-1-AP targets ZFP64 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZFP64 fusion protein Ag10971 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 64 homolog (mouse) |
| Calculated molecular weight | 681 aa, 75 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC041622 |
| Gene Symbol | ZFP64 |
| Gene ID (NCBI) | 55734 |
| RRID | AB_2218826 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NTW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZFP64 (also known as ZNF338) is a member of the Krüppel C2H2-type zinc finger family. ZFP64 is a coactivator of Notch1, mediating mesenchymal cell differentiation. ZFP64 expression was up-regulated in mouse peritoneal macrophages in response to stimulation with the TLR4 ligand LPS with a 2-fold increase. The MLL gene encoding the MLL fusion protein is easily activated by ZFP64. ZFP64 is essential for the continuous production of MLL fusion proteins in blood cancer and produces more cancer cells. Since ZFP64 deletion completely and specifically inhibits MLL fusion protein production and leukemia cascade growth, it is an ideal drug target.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZFP64 antibody 17187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence. | ||
Nat Neurosci Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding | ||
Biol Direct ZFP64 drives glycolysis-mediated stem cell-like properties and tumorigenesis in breast cancer |