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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZFP36L2 Recombinant antibody (85154-4-RR)

    WB analysis using 85154-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFP36L2 Recombinant antibody (85154-4-RR)

    IF Staining of HepG2 using 85154-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L2 antibody (85154-4-RR, Clone: 242776C5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85154-4-RR

    Affinity and Kinetic Characterization of 85154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

ZFP36L2 Recombinant monoclonal antibody

ZFP36L2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85154-4-RR
Clone No.242776C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, Jurkat cells, MCF-7 cells, HepG2 cells, COS-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85154-4-RR targets ZFP36L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag13515

Product name: Recombinant human ZFP36L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 155-497 aa of BC005010

Sequence: KTELCRPFEESGTCKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPSGGASGDLRAFGTRDALHLGFPREPRPKLHHSLSFSGFPSGHHQPPGGLESPLLLDSPTSRTPPPPSCSSASSCSSSASSCSSASAASTPSGAPTCCASAAAAAAAALLYGTGGAEDLLAPGAPCAACSSASCANNAFAFGPELSSLITPLAIQTHNFAAVAAAAYYRSQQQQQQQQQQGLAPPAQPPAPPSATLPAGAAAPPSPPFSFQLPRRLSDSPVFDAPPSPPDSLSDRDSYLSGSLSSGSLSGSESPSLDPGRRLPIFSRLSISDD

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 2
Calculated molecular weight 51 kDa
Observed molecular weight60 kDa
GenBank accession numberBC005010
Gene Symbol ZFP36L2
Gene ID (NCBI) 678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP47974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).

Protocols

Product Specific Protocols
WB protocol for ZFP36L2 antibody 85154-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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