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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFP36L1 Monoclonal antibody (68038-1-Ig)

    WB analysis using 68038-1-Ig (same clone as 68038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ZFP36L1 Monoclonal antibody (68038-1-Ig)

    WB analysis of HSC-T6 using 68038-1-Ig (same clone as 68038-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using ZFP36L1 Monoclonal antibody (68038-1-Ig)

    WB analysis of PC-12 using 68038-1-Ig (same clone as 68038-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 68038-1-Ig (ZFP36L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68038-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZFP36L1 Monoclonal antibody (68038-1-Ig)

    IF Staining of HepG2 using 68038-1-Ig (same clone as 68038-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFP36L1 antibody (68038-1-Ig, Clone: 1H6G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68038-1-PBS in a different storage buffer formulation.

ZFP36L1 Monoclonal antibody, PBS Only

ZFP36L1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68038-1-PBS
Clone No.1H6G6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68038-1-PBS targets ZFP36L1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30469

Product name: Recombinant human ZFP36L1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-338 aa of BC018340

Sequence: MTTTLVSATIFDLSEVLCKGNKMLNYSAPSAGGCLLDRKAVGTPAGGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGGERLLPTQKQPGGGQVNSSRYKTELCRPFEENGACKYGDKCQFAHGIHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRALAGARDLSADRPRLQHSFSFAGFPSAAATAAATGLLDSPTSITPPPILSADDLLGSPTLPDGTNNPFAFSSQELASLFAPSMGLPGGGSPTTFLFRPMSESPHMFDSPPSPQDSLSDQEGYLSSSSSSHSGSDSPTLDNSRRLPIFSRLSISDD

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 36, C3H type-like 1
Calculated molecular weight 338 aa, 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC018340
Gene Symbol ZFP36L1
Gene ID (NCBI) 677
RRIDAB_2918780
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ07352
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

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