Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31404-1-AP targets ZFHX4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35027 Product name: Recombinant human ZFHX4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 400-600 aa of NM_024721 Sequence: QMPKAEVNLGGLSSLVVNTPITSVSLSHSSSESSKMSESKDQENNCERPKESNVLHPNGECPVKSEPTEPGDEDEEDAYSNELDDEEVLGELTDSIGNKDFPLLNQSISPLSSSVLKFIEKGTSSSSATVSDDTEKKKQTAAVRASGSVASNYGISGKDFADASASKDSATAAHPSEIARGDEDSSATPHQHGFTPSTPGT 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger homeobox 4 |
| Calculated molecular weight | 394 kDa |
| Observed molecular weight | 420 kDa |
| GenBank accession number | NM_024721 |
| Gene Symbol | ZFHX4 |
| Gene ID (NCBI) | 79776 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UP3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ZFHX4 protein (Zinc Finger Homeobox Protein 4) belongs to the zinc finger transcription factor family. Its structure contains multiple zinc finger motifs and a homeobox domain. It mainly regulates the expression of specific genes by binding to DNA. This protein plays a crucial role in the development and differentiation of the nervous system, participating in the precise regulation of neuronal gene expression. In the adult stage, it is also related to muscle regeneration and the regulation of tumor occurrence (such as glioblastoma and melanoma), and may act as a transcriptional repressor to influence the balance of cell proliferation and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZFHX4 antibody 31404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |