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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human breast cancer using 20157-1-AP (same clone as 20157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human prostate cancer using 20157-1-AP (same clone as 20157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human prostate cancer using 20157-1-AP (same clone as 20157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZFHX3 Polyclonal antibody (20157-1-AP)

    IHC staining of human breast cancer using 20157-1-AP (same clone as 20157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20157-1-AP (ZFHX3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZFHX3 Polyclonal antibody (20157-1-AP)

    IF Staining of HeLa using 20157-1-AP (same clone as 20157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFHX3 antibody (20157-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZFHX3 Polyclonal antibody (20157-1-AP)

    IF Staining of MCF-7 using 20157-1-AP (same clone as 20157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZFHX3 antibody (20157-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20157-1-PBS in a different storage buffer formulation.

ZFHX3 Polyclonal antibody, PBS Only

ZFHX3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 20157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

C16orf47, ATBT, AT-binding transcription factor 1, ATBF1, AT motif-binding factor 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20157-1-PBS targets ZFHX3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14080

Product name: Recombinant human ZFHX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 921-1270 aa of BC029653

Sequence: QLVSEELMNLGESFIQTNDPSLKLFQCAVCNKFTTDNLDMLGLHMNVERSLSEDEWKAVMGDSYQCKLCRYNTQLKANFQLHCKTDKHVQKYQLVAHIKEGGKANEWRLKCVAIGNPVHLKCNACDYYTNSLEKLRLHTVNSRHEASLKLYKHLQQHESGVEGESCYYHCVLCNYSTKAKLNLIQHVRSMKHQRSESLRKLQRLQKGLPEEDEDLGQIFTIRRCPSTDPEEAIEDVEGPSETAADPEELAKDQEGGASSSQAEKELTDSPATSKRISFPGSSESPLSSKRPKTAEEIKPEQMYQCPYCKYSNADVNRLRVHAMTQHSVQPMLRCPLCQDMLNNKIHLQLH

相同性解析による交差性が予測される生物種
Full Name zinc finger homeobox 3
Calculated molecular weight 3703 aa, 404 kDa
GenBank accession numberBC029653
Gene Symbol ZFHX3
Gene ID (NCBI) 463
RRIDAB_3669324
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15911
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc finger homeobox protein 3 (ZFHX3) encodes a transcription factor which contains four homeodomains and seventeen zinc fingers. The ZFHX3 transcription factor appears to regulate myogenic and neuronal differentiation. Although the function of ZFHX3 in cardiac tissue is unknown, it is expressed in mouse hearts. (PMID: 26267381)

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