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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZER1 Monoclonal antibody (68258-1-Ig)

    WB analysis of Jurkat using 68258-1-Ig (same clone as 68258-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using ZER1 Monoclonal antibody (68258-1-Ig)

    WB analysis of LNCaP using 68258-1-Ig (same clone as 68258-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZER1 Monoclonal antibody (68258-1-Ig)

    IF Staining of HepG2 using 68258-1-Ig (same clone as 68258-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZER1 antibody (68258-1-Ig, Clone: 1H9G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68258-1-PBS in a different storage buffer formulation.

ZER1 Monoclonal antibody, PBS Only

ZER1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68258-1-PBS
Clone No.1H9G7

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68258-1-PBS targets ZER1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10040

Product name: Recombinant human ZER1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 324-766 aa of BC052563

Sequence: RPLQFLGLFENSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR

相同性解析による交差性が予測される生物種
Full Name zer-1 homolog (C. elegans)
Calculated molecular weight 766 aa, 88 kDa
Observed molecular weight 80-88 kDa
GenBank accession numberBC052563
Gene Symbol ZER1
Gene ID (NCBI) 10444
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ7Z7L7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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