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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZEB1 Recombinant antibody (85729-1-RR)

    WB analysis using 85729-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZEB1 Recombinant antibody (85729-1-RR)

    WB analysis of HepG2 using 85729-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ZEB1 Recombinant antibody (85729-1-RR)

    WB analysis of NIH/3T3 using 85729-1-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZEB1 Recombinant antibody (85729-1-RR)

    WB analysis using 85729-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85729-1-RR (ZEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Recombinant antibody (85729-1-RR)

    IHC staining of human colon cancer using 85729-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85729-1-RR (ZEB1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZEB1 Recombinant antibody (85729-1-RR)

    IHC staining of human colon cancer using 85729-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85729-1-RR (ZEB1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZEB1 Recombinant antibody (85729-1-RR)

    IF Staining of HeLa using 85729-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ZEB1 Recombinant antibody (85729-1-RR)

    IF Staining of NIH/3T3 using 85729-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ZEB1 antibody (85729-1-RR, Clone: 243139F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85729-1-RR

    Affinity and Kinetic Characterization of 85729-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85729-1-RR against Human ZEB1 were performed. The affinity constant is 0.693 nM.

ZEB1 Recombinant monoclonal antibody

ZEB1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85729-1-RR
Clone No.243139F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse colon tissue, NIH/3T3 cells, COLO 320 cells, rat colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85729-1-RR targets ZEB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16204

Product name: Recombinant human ZEB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 391-740 aa of BC112392

Sequence: MVQAVVLPTVGLVSPISINLSDIQNVLKVAVDGNVIRQVLENNQANLASKEQETINASPIQQGGHSVISAISLPLVDQDGTTKIIINYSLEQPSQLQVVPQNLKKENPVATNSCKSEKLPEDLTVKSEKDKSFEGGVNDSTCLLCDDCPGDINALPELKHYDLKQPTQPPPLPAAEAEKPESSVSSATGDGNLSPSQPPLKNLLSLLKAYYALNAQPSAEELSKIADSVNLPLDVVKKWFEKMQAGQISVQSSEPSSPEPGKVNIPAKNNDQPQSANANEPQDSTVNLQSPLKMTNSPVLPVGSTTNGSRSSTPSPSPLNLSSSRNTQGYLYTAEGAQEEPQVEPLDLSL

相同性解析による交差性が予測される生物種
Full Name zinc finger E-box binding homeobox 1
Calculated molecular weight 1125 aa, 124 kDa
Observed molecular weight 190-210 kDa
GenBank accession numberBC112392
Gene Symbol ZEB1
Gene ID (NCBI) 6935
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP37275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.

Protocols

Product Specific Protocols
IF protocol for ZEB1 antibody 85729-1-RRDownload protocol
IHC protocol for ZEB1 antibody 85729-1-RRDownload protocol
WB protocol for ZEB1 antibody 85729-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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