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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis using 24046-1-AP (same clone as 24046-1-PBS)

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse brain using 24046-1-AP (same clone as 24046-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of SH-SY5Y using 24046-1-AP (same clone as 24046-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse liver using 24046-1-AP (same clone as 24046-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse brain using 24046-1-AP (same clone as 24046-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    WB analysis of mouse skeletal muscle using 24046-1-AP (same clone as 24046-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human testis using 24046-1-AP (same clone as 24046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human liver using 24046-1-AP (same clone as 24046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IHC staining of human testis using 24046-1-AP (same clone as 24046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ZDHHC9 Polyclonal antibody (24046-1-AP)

    IF Staining of SH-SY5Y using 24046-1-AP (same clone as 24046-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24046-1-PBS in a different storage buffer formulation.

ZDHHC9 Polyclonal antibody, PBS Only

ZDHHC9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24046-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CGI-89, CXorf11, DHHC 9, DHHC9, DHHC-9

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24046-1-PBS targets ZDHHC9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20881

Product name: Recombinant human ZDHHC9 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 87-364 aa of BC006200

Sequence: SFSDPGVIPRALPDEAAFIEMEIEATNGAVPQGQRPPPRIKNFQINNQIVKLKYCYTCKIFRPPRASHCSICDNCVERFDHHCPWVGNCVGKRNYRYFYLFILSLSLLTIYVFAFNIVYVALKSLKIGFLETLKETPGTVLEVLICFFTLWSVVGLTGFHTFLVALNQTTNEDIKGSWTGKNRVQNPYSHGNIVKNCCEVLCGPLPPSVLDRRGILPLEESGSRPPSTQETSSSLLPQSPAPTEHLNSNEMPEDSSTPEEMPPPEPPEPPQEAAEAEK

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 9
Calculated molecular weight 41 kDa
Observed molecular weight 38-48 kDa
GenBank accession numberBC006200
Gene Symbol ZDHHC9
Gene ID (NCBI) 51114
RRIDAB_2879415
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9Y397
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

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