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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZDHHC8 Recombinant monoclonal antibody (86572-2-RR)

    WB analysis of HeLa using 86572-2-RR (same clone as 86572-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 86572-2-RR (ZDHHC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86572-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZDHHC8 Recombinant monoclonal antibody (86572-2-RR)

    WB analysis using 86572-2-RR (same clone as 86572-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86572-2-RR (ZDHHC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86572-2-PBS in a different storage buffer formulation.

  • Affinity of 86572-2-RR

    Affinity of 86572-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86572-2-RR against Human ZDHHC8 were performed. The affinity constant is 0.915 nM.

ZDHHC8 Recombinant monoclonal antibody, PBS Only

ZDHHC8 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86572-2-PBS
Clone No.251438A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

DHHC 8, DHHC8, DHHC-8, EC:2.3.1.225, KIAA1292

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86572-2-PBS targets ZDHHC8 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag26028

Product name: Recombinant human ZDHHC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 274-430 aa of BC053544

Sequence: LLDRAAPLKVKLSDNGLKAGLGRSKSKGSLDRLDEKPLDLGPPLPPKIEAGTFSSDLQTPRPGSAESALSVQRTSPPTPAMYKFRPAFPTGPKVPFCGPGEQVPGPDSLTLGDDSIRSLDFVSEPSLDLPDYGPGGLHAAYPPSPPLSASDAFSGAL

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 8
Calculated molecular weight 83 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC053544
Gene Symbol ZDHHC8
Gene ID (NCBI) 29801
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULC8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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