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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZDHHC7 Polyclonal antibody (25985-1-AP)

    WB analysis of PC-3 using 25985-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25985-1-AP (ZDHHC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZDHHC7 Polyclonal antibody (25985-1-AP)

    WB analysis of MCF-7 using 25985-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25985-1-AP (ZDHHC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ZDHHC7 Polyclonal antibody (25985-1-AP)

    WB analysis of PC-3 using 25985-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25985-1-AP (ZDHHC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ZDHHC7 Polyclonal antibody (25985-1-AP)

    WB analysis of MCF-7 using 25985-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25985-1-AP (ZDHHC7 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

ZDHHC7 Polyclonal antibody

ZDHHC7 Polyclonal Antibody for WB, ELISA
Cat No. 25985-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DHHC 7, Palmitoyltransferase ZDHHC7, ZDHHC7, Zinc finger protein 370, ZNF370

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25985-1-AP targets ZDHHC7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23185

Product name: Recombinant human ZDHHC7 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 279-345 aa of BC018772

Sequence: IHSICNDETEIERLKSEKPTWERRLRWEGMKSVFGGPPSLLWMNPFVGFRFRRLPTRPRKGGPEFSV

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 7
Calculated molecular weight 345 aa, 39 kDa
Observed molecular weight 45-49 kDa
GenBank accession numberBC018772
Gene Symbol ZDHHC7
Gene ID (NCBI) 55625
RRIDAB_2880320
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZDHHC7 antibody 25985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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