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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZDHHC5 Recombinant antibody (84803-4-RR)

    WB analysis using 84803-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZDHHC5 Recombinant antibody (84803-4-RR)

    IP experiment of HEK-293 using 84803-4-RR

    IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZDHHC5 Recombinant antibody (84803-4-RR)

    IHC staining of human ovary cancer using 84803-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZDHHC5 Recombinant antibody (84803-4-RR)

    IHC staining of human ovary cancer using 84803-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84803-4-RR

    Affinity and Kinetic Characterization of 84803-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

ZDHHC5 Recombinant monoclonal antibody

ZDHHC5 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 84803-4-RR
Clone No.242192A4
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84803-4-RR targets ZDHHC5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15022

Product name: Recombinant human ZDHHC5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 313-662 aa of BC026967

Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 5
Calculated molecular weight 715 aa, 78 kDa
Observed molecular weight 75-77 kDa
GenBank accession numberBC026967
Gene Symbol ZDHHC5
Gene ID (NCBI) 25921
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9C0B5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Protocols

Product Specific Protocols
IHC protocol for ZDHHC5 antibody 84803-4-RRDownload protocol
IP protocol for ZDHHC5 antibody 84803-4-RRDownload protocol
WB protocol for ZDHHC5 antibody 84803-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Virol

Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation

Authors - Qisheng Qian
View Article
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