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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis using 21627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using ZDHHC15 Polyclonal antibody (21627-1-AP)

    WB analysis of SMMC-7721 using 21627-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 21627-1-AP (ZDHHC15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZDHHC15 Polyclonal antibody

ZDHHC15 Polyclonal Antibody for WB, ELISA
Cat No. 21627-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

MRX91, EC:2.3.1.225, EC:2.3.1.-, DHHC-15, DHHC 15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, SMMC-7721 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

21627-1-AP targets ZDHHC15 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16152

Product name: Recombinant human ZDHHC15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-337 aa of BC103982

Sequence: VSRNKTTLEAFCTPVFTSGPEKNGFNLGFIKNIQQVFGDKKKFWLIPIGSSPGDGHSFPMRSMNESQNPLLANEETWEDNEDDNQDYPEGSSSLAVETET

相同性解析による交差性が予測される生物種
Full Name zinc finger, DHHC-type containing 15
Calculated molecular weight 337 aa, 39 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC103982
Gene Symbol ZDHHC15
Gene ID (NCBI) 158866
RRIDAB_2878892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZDHHC15 antibody 21627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Sci

Adipocyte-Derived Palmitic Acid Promotes Breast Cancer Malignancy via ZDHHC15-Mediated S-Palmitoylation of PPARγ.

Authors - Geon Ho Moon
View Article
IP

Mol Cell Endocrinol

ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells

Authors - Shanmeizi Zhao
View Article
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