Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26023-1-AP targets ZCCHC13 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZCCHC13 fusion protein Ag22050 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, CCHC domain containing 13 |
| Calculated molecular weight | 166 aa, 18 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC021176 |
| Gene Symbol | ZCCHC13 |
| Gene ID (NCBI) | 389874 |
| RRID | AB_3669517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WW36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZCCHC13 (zinc-finger CCHC-type containing 13) is a member of a family of zinc-finger CCHC-type containing DNA/RNA-binding proteins that are involved in mRNA transcription and protein translation. Downregulation of ZCCHC13 was shown to be associated with spermatogenesis failure in patients with non-obstructive azoospermia (NOA)(PMID: 29531833). It is reported that ZCCHC13 overexpression might be related to global DNA hypomethylation and promote hepatocellular carcinoma (HCC)(PMID: 23891108).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZCCHC13 antibody 26023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |