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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ZC3HAV1L Polyclonal antibody

ZC3HAV1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20944-1-AP

Synonyms

C7orf39, ZC3HAV1L

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 20944-1-AP

    WB analysis of Jurkat using 20944-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20944-1-AP

    WB analysis of HeLa using 20944-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20944-1-AP

    WB analysis of HepG2 using 20944-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SW 1990 using 20944-1-AP

    WB analysis of SW 1990 using 20944-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20944-1-AP

    IHC staining of human kidney using 20944-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20944-1-AP (ZC3HAV1L Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 20944-1-AP

    IHC staining of human kidney using 20944-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20944-1-AP (ZC3HAV1L Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, SW 1990 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20944-1-AP is ZC3HAV1L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3HAV1L fusion protein Ag15052 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type, antiviral 1-like
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC008842
Gene symbol ZC3HAV1L
Gene ID (NCBI) 92092
RRIDAB_2878771
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZC3HAV1L antibody 20944-1-APDownload protocol
IHC protocol for ZC3HAV1L antibody 20944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols