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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis of HUVEC using 25624-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis using 25624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis of HUVEC using 25624-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis of mouse brain using 25624-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis of mouse brain using 25624-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using ZC3H7B Polyclonal antibody (25624-1-AP)

    WB analysis of HUVEC using 25624-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ZC3H7B Polyclonal antibody

ZC3H7B Polyclonal Antibody for WB, ELISA
Cat No. 25624-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA1031, Rotavirus 'X'-associated non-structural protein, RoXaN, Zinc finger CCCH domain-containing protein 7B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHUVEC cells, A549 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25624-1-AP targets ZC3H7B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22555

Product name: Recombinant human ZC3H7B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 169-460 aa of BC152558

Sequence: KAYKRPQELETFSLLSNGTAAGVADQGTSNGLGSIDDIETDCYVDPRGSPALLPSTPTMPLFTHVLDLLAPLDSSRTLPSTDSLDDFSDGDVFGPELDTLLDSLSLVQGGLSGSGVPSELPQLIPVFPGGTPLLPPVVGGSIPVSSPLPPASFGLVMDPSKKLAASVLDALDPPGPTLDPLDLLPYSETRLDALDSFGSTRGSLDKPDSFMEETNSQDHRPTSGAQKPAPSPEPCMPNTALLIKNPLAATHEFKQACQLCYPKTGPRAGDYTYREGLEHKCKRDILLGRLRS

相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 7B
Calculated molecular weight 993 aa, 112 kDa
Observed molecular weight 111 kDa
GenBank accession numberBC152558
Gene Symbol ZC3H7B
Gene ID (NCBI) 23264
RRIDAB_2880166
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZC3H7B antibody 25624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Acta Neuropathol

Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.

Authors - Di Hu
View Article
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