Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 3 publications below |
Product Information
20041-1-AP targets ZC3H4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type containing 4 |
| Calculated molecular weight | 140 kDa |
| Observed molecular weight | 130-140 kDa |
| GenBank accession number | NM_015168 |
| Gene Symbol | ZC3H4 |
| Gene ID (NCBI) | 23211 |
| RRID | AB_10667456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IHC protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IF protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression | ||
FASEB J Silica-induced initiation of circular ZC3H4 RNA/ZC3H4 pathway promotes the pulmonary macrophage activation. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. | ||
Lab Invest Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition. | ||
Toxicol Appl Pharmacol ZC3H4 promotes pulmonary fibrosis via an ER stress-related positive feedback loop. | ||
Environ Toxicol Pharmacol ZC3H4 mediates silica-induced EndoMT via ER stress and autophagy. |