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  • Featured Product
  • KD/KO Validated

ZC3H15 Polyclonal antibody

ZC3H15 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26241-1-AP

Synonyms

DFRP1, HT010, LEREPO4, MSTP012, PP730, ZC3H15

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 26241-1-AP

    WB analysis of K-562 using 26241-1-AP

    WB result of ZC3H15 antibody (26241-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H15 transfected K-562 cells.

  • WB analysis of HeLa using 26241-1-AP

    WB analysis of HeLa using 26241-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 26241-1-AP

    WB analysis of HL-60 using 26241-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 26241-1-AP

    WB analysis of K-562 using 26241-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26241-1-AP (ZC3H15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • IHC staining of human cervical cancer using 26241-1-AP

    IHC staining of human cervical cancer using 26241-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26241-1-AP (ZC3H15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H15 antibody included
  • IF Staining of U2OS using 26241-1-AP

    IF Staining of U2OS using 26241-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ZC3H15 antibody (26241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 26241-1-AP

    IF Staining of HeLa using 26241-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26241-1-AP (ZC3H15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H15 Ready-To-Use IHC Kit. ZC3H15 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

26241-1-AP targets ZC3H15 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZC3H15 fusion protein Ag24091 相同性解析による交差性が予測される生物種
Full Name zinc finger CCCH-type containing 15
Observed molecular weight 50-55 kDa
GenBank accession numberBC021102
Gene symbol ZC3H15
Gene ID (NCBI) 55854
RRIDAB_2880441
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZC3H15 antibody 26241-1-APDownload protocol
IHC protocol for ZC3H15 antibody 26241-1-APDownload protocol
IF protocol for ZC3H15 antibody 26241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols