Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse spleen tissue, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human urothelial carcinoma tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
25009-1-AP targets ZC3H12A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13877 Product name: Recombinant human ZC3H12A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 339-599 aa of BC005001 Sequence: ANALLSPPRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAPSGGSGSSFGPTDWLPQTLDSLPYVSQDCLDSGIGSLESQMSELWGVRGGGPGEPGPPRAPYTGYSPYGSELPATAAFSAFGRAMGAGHFSVPADYPPAPPAFPPREYWSEPYPLPPPTSVLQEPPVQSPGAGRSPWGRADSLAKEQASVYTKLCGVFPPHLVEAVMGRFPQLLDPQQLAAEILSYKSQHPSE 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger CCCH-type containing 12A |
| Calculated molecular weight | 599 aa, 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC005001 |
| Gene Symbol | ZC3H12A |
| Gene ID (NCBI) | 80149 |
| RRID | AB_2879844 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5D1E8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZC3H12A antibody 25009-1-AP | Download protocol |
| IP protocol for ZC3H12A antibody 25009-1-AP | Download protocol |
| WB protocol for ZC3H12A antibody 25009-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov CD70-Targeted Allogeneic CAR T-Cell Therapy for Advanced Clear Cell Renal Cell Carcinoma | ||
Chin Med Based on the gut-heart axis: Polygonum capitatum improves atherosclerosis by modulating gut microbiota and TMAO, supporting MCPIP1/p53-associated endothelial protection.
| ||
Int Immunopharmacol MCPIP1 modulates host antifungal immunity and exacerbates lethal Candida albicans infection. | ||
Int Immunopharmacol MCPIP1 alleviates inflammatory response through inducing autophagy in Aspergillus fumigatus keratitis
| ||
Mol Med Rep MCPIP1 alleviates depressive‑like behaviors in mice by inhibiting the TLR4/TRAF6/NF‑κB pathway to suppress neuroinflammation | ||
Retrovirology Increased expression of CDKN1A/p21 in HIV-1 controllers is correlated with upregulation of ZC3H12A/MCPIP1. |