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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    WB analysis of HEK-293 using 24544-1-AP (same clone as 24544-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24544-1-AP (ZBTB8A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24544-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    IP experiment of HEK-293 using 24544-1-AP (same clone as 24544-1-PBS)

    IP result of anti-ZBTB8A (IP:24544-1-AP, 3ug; Detection:24544-1-AP 1:300) with HEK-293 cells lysate 920ug. This data was developed using the same antibody clone with 24544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ZBTB8A Polyclonal antibody (24544-1-AP)

    IF Staining of HEK-293 using 24544-1-AP (same clone as 24544-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 24544-1-AP (ZBTB8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24544-1-PBS in a different storage buffer formulation.

ZBTB8A Polyclonal antibody, PBS Only

ZBTB8A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24544-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24544-1-PBS targets ZBTB8A in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21588

Product name: Recombinant human ZBTB8A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 106-296 aa of BC015239

Sequence: QMTDVISVCKTFIKSSLDISEKEKDRYFSLSDKDANSNGVERSSFYSGGWQEGSSSPRSHLSPEQGTGIISGKSWNKYNYHPASQKNTQQPLAKHEPRKESIKKTKHLRLSQPSEVTHYKSSKREVRTSDSSSHVSQSEEQAQIDAEMDSTPVGYQYGQGSDVTSKSFPDDLPRMRFKCPYCTHVVKRKAD

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 8A
Calculated molecular weight 441 aa, 50 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC015239
Gene Symbol ZBTB8A
Gene ID (NCBI) 653121
RRIDAB_2879599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BR9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZBTB8A, also named as Zinc finger and BTB domain-containing protein 8A, is a 441 amino acid protein, which may be involved in transcriptional regulation. ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis. The calcualted molecular weight of ZBTB8A is 50 kDa, but modified ZBTB8A is about 55-60 kDa.

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