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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZBTB7A Polyclonal antibody (30658-1-AP)

    WB analysis using 30658-1-AP (same clone as 30658-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30658-1-AP (ZBTB7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZBTB7A Polyclonal antibody (30658-1-AP)

    WB analysis using 30658-1-AP (same clone as 30658-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30658-1-AP (ZBTB7A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ZBTB7A Polyclonal antibody (30658-1-AP)

    IHC staining of human lung using 30658-1-AP (same clone as 30658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30658-1-AP (ZBTB7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using ZBTB7A Polyclonal antibody (30658-1-AP)

    IHC staining of human lung using 30658-1-AP (same clone as 30658-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30658-1-AP (ZBTB7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ZBTB7A Polyclonal antibody (30658-1-AP)

    IF Staining of MCF-7 using 30658-1-AP (same clone as 30658-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB7A antibody (30658-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZBTB7A Polyclonal antibody (30658-1-AP)

    IF Staining of HeLa using 30658-1-AP (same clone as 30658-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBTB7A antibody (30658-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30658-1-PBS in a different storage buffer formulation.

ZBTB7A Polyclonal antibody, PBS Only

ZBTB7A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30658-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Factor binding IST protein 1, FBI 1, FBI1, FBI-1, HIV 1 1st binding protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30658-1-PBS targets ZBTB7A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31077

Product name: Recombinant human ZBTB7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-584 aa of NM_015898

Sequence: VHTGLRPYQCDSCCKTFVRSDHLHRHLKKDGCNGVPSRRGRKPRVRGGAPDPSPGATATPGAPAQPSSPDARRNGQEKHFKDEDEDEDVASPDGLGRLNVAGAGGGGDSGGGPGAATDGNFTAGLA

相同性解析による交差性が予測される生物種
Full Name zinc finger and BTB domain containing 7A
Calculated molecular weight 61 kDa
Observed molecular weight61-70 kDa
GenBank accession numberNM_015898
Gene Symbol ZBTB7A
Gene ID (NCBI) 51341
RRIDAB_3669747
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95365
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa.

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